The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). FilterMax F5 Multi-Mode Microplate Reader The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence,. The following multi-step binary elution gradient, based on an water as phase A and acetonitrile as phase B, both containing 0. ScanLater Western Blot Detection System. 0. Absorbance Detection Comparison Table. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. SpectraMax Glo Steady-Luc Reporter Assay Kit. MetaFluor Software. Z’-LYTE® Compatible Microplate Reader Documentation Version No. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. The solution was incubated for 15 min and fluorescence measured on a SpectraMax microplate reader (Molecular Devices) with an. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. FilterMax F5 Multi-mode microplate reader. ScanLater Western Blot Detection System. A Vision for the Future With TTP LabTech. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). The OD 600 was plotted against time using Graphpad Prism 8. Synergy HTX and Synergy LX. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. CloneMatrix. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). , Shanghai, China) and microplate reader (FilterMax F5 Multi-Mode Microplate Reader, LLC, Molecular Devices, San Jose, CA, United States) at a wavelength of 450 nm. 09 CAD. BMG POLARstar Microplate Reader, 115-230V, BMG Labtech (CAT#: STEM-SPO-0061-YJL) Perkin Elmer Victor3 1420 Multilabel Counter, 110-240V, 3 Amps, PerkinElmer (CAT#: STEM-SPO. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. Cells were harvested for luciferase assay 48 h after transfection. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The experiment was carried out with four biological replicates and two technical replicates. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. Caspase 3/7 activity was performed as previously described [8,9,10,20]. 5 mP. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Neurotransmitter Transporter Uptake Assay Kit. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. Methods for MycoAlert and MycoAlert PLUS Assays are basically the same, except for the concentration range of assay control used, and are detailed below. It allows the user to choose between monochromator-based optics for flexibility or filter-based optics for sensitivity. 1 (GraphPad Software Inc. In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. Plate Formats:. 4 million by 2027; it is expected to grow at a CAGR of 3. The optical performance is comparable to a top-of-the-line dedicated Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Optical System: High energy xenon flash lamp. 5 ml 0. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. Each kit was used on the same day that its reagents were reconstituted. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. , Sunnyvale, CA, USA). This minor release branch supports the B2100 (A109) hardware product until the product's End of Software. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). SoftMax® Pro Microplate Data Analysis Software provides integrated instrument control and data analysis. 3. After 24 h, the. Neurotransmitter Transporter Uptake Assay Kit. 5 μg/ml) in the dark for 1 h. 2 Pages. 3 Pages. At day 4 P. 8. , San Diego, CA, USA). 4. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). ; Page 2 For research use only. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. Load prepared PicoGreen plate (step C. 3 Pages. SpectraMax Gemini XPS/EM Microplate Readers. In addition, the instrument features linear and orbital shaking, plus temperature. 425 or. Luciferase activity was quantified by the Dual-Glo Luciferase Assay System (Promega) as per the manufacturer’s instructions on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices). Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. The supernatant was taken and 0. Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Ease of use. Create an experiment based on the protocol. They provide absorbance, fluorescence, glow luminescence, and other read modes for. Neurotransmitter Transporter Uptake Assay Kit. Add to cart. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. GenePix 4300/4400 Microarray Scanner. Synergy™ H1 Hybrid Multi-Mode Microplate Reader, Wavelength Range: 200 nm to 999 nm, BioTek Instruments (CAT#:. 00 USD. The unique patented design ensures precise performance and sensitivity across all detection modes. Z’-LYTE® Compatible Microplate Reader Documentation Version No. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. 2. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Gently pull the plastic tab protruding from the microplate chamber door. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. Measurements of Caspase-3/7 Activity. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. SP Bel-Art Petri Dish Plate Reader, Bel-Art Products, a part of SP . ABS Test results. Discover Precision and Reliability with Our Featured General Laboratory Instruments - Thermo Forma 3110 Water Jacketed CO2 Incubator, Thermo ULT185-5-V -80 Freezer, Filtermax F5 Multimode Microplate Reader, Spectramax M5e, Thermo Napco CO2 Incubator, and XS 64 Balance. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Yes, the M5 does have a limited slip. SoftMax Pro GxP Compliance Software. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. CloneMatrix. ScanLater Western Blot Detection System. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. 7. Not for use in diagnostic procedures. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. 4 Pages. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. ” e. After adding 100 μl of new ice-cold PBS to the cells, fluorescent intensity was immediately determined using the fluorescent plate reader (FilterMax F3&F5 Multi-Mode Microplate Reader, Molecular Devices). Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 3 Pages. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. 3 Pages. Table of Contents. This can increase throughput and efficiency, thus saving time and space in the lab. The amount of colorimetric product was continuously monitored for 120 min. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. F5 has made an exception to this policy for BIG-IP 13. To read the absorbance, a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA 95134, USA) with a wavelength of 595 nm was used. The colorimetric signal development was stopped after 10 minutes. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The mixture was then boiled for 10 min. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. SpectraMax Glo Steady-Luc Reporter Assay Kit. 2. 00The microplate reader market was valued at US$ 534. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. F3/F5 Multi-Mode Microplate Readers. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. J. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 1 Software Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. 4 Pages. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between failure (MTBF) of 20,000 hours with 97. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Bio-Tek Microplate Reader Synergy HT $8,995. Vertical Jump. com A. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. Hit enter to search or ESC to close. 005 OD. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). All experiments were performed in triplicate. 9. - September 14, 2010 - Molecular Devices, Inc. 50/50. Laser-aided direct metal deposition of metals and alloys. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. With optimized reagents, option to operate the. prt Read the plate and then save the experiment. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. 4 Pages. 40266 Email: [email protected]™ F3 and F5 Multimode Microplate Readers, Molecular Devices. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. You can also contact Technical Support by email. MICROPLATE READERS Multi-Mode Readers. All experiments were performed in triplicate. HERM LB 500 with NaI detector Radio HPLC Detector. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). 2 Pages. This instrument has passed specific verification criteria. 2 Pages. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. 6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. Vascular. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. ScanLater Western Blot Detection System. Plaque reduction. Trinean Dropsense 96 Polychromatic. Cells were seeded in 24-well plates and cultured. » FilterMax™ F5 Multi-Mode Microplate Reader » SpectraMax L Microplate Luminometer ATP + Luciferin + 0 2 Oxyluciferin + AMP + PPi + CO 2 + Light Mg2+ Luciferase Benefits • Sensitive, reliable detection of mycoplasma contamination. Match case Limit results 1 per page. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 3 Pages. A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. 00 USD. Easily accessible six-position filter slides enable wavelength selection from 340 to 650nm. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. FilterMax F5 Multi-Mode Microplate Reader. Statistical analysis was performed using. SpectraMax iD5. 7°C (gradient heating design). performs within its specifications. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. FilterMax F5 Multi-Mode Microplate Reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 2. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. BMG Labtech - FLUOSTAR OMEGA. The values of luminescence are directly proportional to the number of viable cells. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. 00. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. 6% from 2020 to 2027. Recommended Optics The lysates were incubated in caspase-3 substrate Ac-DEVD-pNA at 37 °C. Read Review (1)The mixture were then centrifuged at 1000 g for 5 min. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 3 Pages. The values of luminescence are directly proportional to the number of viable cells. . F3 F5 F3-SC F5-SC F3 F5. Devices FilterMax Multi-Mode Microplate Readers. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular. FilterMax F3 & F5 Multi-Mode Microplate Readers from. Immunoblot analysis was performed with pooled serum (diluted 1:500) separated by SDS-PAGE under non-reducing conditions and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). SpectraMax M-series Microplate readers. 3 Pages. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Catalog. Each condition was performed in. F3/F5 Multi-Mode Microplate Readers Fluorescent signals from the same assay plate were measured on the SpectraMax iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. MetaFluor Software Brochure. DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. Statistical analysis was performed using. org) with optimized instrument settings b. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). SpectraMax® iD5 Multi-Mode Microplate Reader (Molecular Devices cat. The results are expressed as cell viabilities (%) based on the control condition. Neurotransmitter Transporter Uptake Assay Kit. 5% (v/v) to avoid toxicity. Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 7. shopping cart 0 items $0. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. ScanLater Western Blot Detection System. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). 5 million in 2019 and is projected to reach US$ 704. Citations: Dual monochromators increase performance and assay flexibility. FilterMax F5 Multi-Mode Microplate Reader. A blank sample was prepared by mixing. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. Protein contamination can be measured at 280 nm. The experiment was carried out with two biological replicates and three technical replicates. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. The fluorescent signals were measured at 10 minutes and one hour after the Stoplight Red substrateFilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) 12. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 3 Pages. The Hidex Sense is a compact, state-of-the-art, multi-mode microplate reader which provides a powerful and flexible platform for performing any kind of assay without limitations. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax iD3 and iD5 Multi-Mode Microplate Readers;. SpectraMax Quant dsDNA Assay Kits. Download Table of Contents. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. MD, MPH, Milton J. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. shopping cart 0 items $0. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. ” e. Turn on the power switch on the back of the instrument. 2 Pages. Molecular Devices technical support is available by phone and e-mail. Molecular Devices Microplate reader VersaMax $1,495. Page 38 | Getting Started Overview The Cytation 5 is a hybrid multi-mode microplate reader. ] Example of document to evaluate if the reader . Place plate in the GloMax® Instrument and read. SpectraMax Mini Multi-Mode Microplate Reader. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. The measurement can be performed using a variety of detection technologies: the. FilterMax Multi-Mode Microplate Readers Functionality FilterMax F3 FilterMax F5 Absorbance (ABS ) Visible 4 4 Absorbance (ABS) UV 4 Fluorescence intensity (FI) top read 4 4 Fluorescence intensity (FI) bottom read 4 Luminescence (LUM) glow 4 4 Fluorescence polarization (FP) 4 Time-Resolved fluorescence (TRF) 4 Shaking 4 4 Temperature. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. 4 fmol/100 μl fluorescein in Fluorescence Intensity-Top Read Mode, 0. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Neurotransmitter Transporter Uptake Assay Kit. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Filters: Excitation and emission slides for 4 filters each. 05); b Evaluation of the ATP levels of T. MDA is one of the final products of LPO. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 0´ 1 4 4. GFP expression differences. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. Bioprocess Equipment and Technology. Match case Limit results 1 per page. Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. Multi-mode Microplate Readers. 00. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. SpectraMax iD5. Samples (180 μl) were stained with 20 μl of Nile red (2. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. 380 or. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. Turn on the power to the FilterMax F5. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe parasite viability was determined by resazurin assay (0. Caspase-3 Activity. When recombinant supernatants were tested for the presence of Il10, only values within the linear range of. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. MS-3 Laser Bar Code Reader. After incubation, each microplate was analyzed for absorbance reading. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Click “Eject Plate Carrier. Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. Molecular Devices FilterMax F5 User Manual (49 pages) Multi-Mode Microplate Readers. How to Get Multi-Day Kinetic Data. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. Share. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. In the list below the slide number 21, select the positions where the emission filters are installedAfter 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. 2 μM ATP standard was. FilterMax F3 & F5 Multi-Mode Microplate Readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Users can program endpoint, kinetic, multiple wavelength, linear scan, and area scan measurement types. . com B. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. 100 fmol 384-well (75 µL) 0. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). Readers ®Examples: Monochromators such as the SpectraMax i3 Multi-Mode Microplate Reader Detection Platform or filter based systems like the FilterMax™ F3 and F5 Multi-Mode Microplate Readers Luminescence Principle The total amount of light emitted is collected Plate specs White plates to reflect all the light to the detectorSpectraMax i3/i3x Multi-Mode Microplate Reader HeLa cells Cell viability assay SoftMax Pro Software Simplified homogenous experimental protocol The EarlyTox cell viability assay kits apply a no-wash, homogeneous protocol that simplifies the workflow and reduces the data variability typically encountered with multiple experimental steps. SpectraMax Glo Steady-Luc Reporter Assay Kit. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular devices, CA, USA). Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#: STEM-LE-0509-LC) Mithras LB 940 Multimode Microplate Reader, 200-1000 nm Spectral Range Absorbance, BERTHOLD TECHNOLOGIES GmbH & Co. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The measured temperature can be used to provide feedback. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. Caspase 3/7 activity was measured according to previously established methods [11,12,13,15,16]. Microplate readers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. 25. 4. The following document is intended to demonstrate setup of this instrument. MetaMorph NX. 425 or. 50/50. FilterMax F3 & F5 Microplate Readers. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in.